FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75432
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.11666136387739952No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.11135857461024498No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.1073814826598791No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.1073814826598791No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.10075299607593595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT255.2568794E-10295.01
TCGCCCT157.2136154E-6295.01
CGAACCT350.0295.01
ACGCCTA750.0295.02
ACGCCCT108.4493763E-4295.01
GCGACCT206.146911E-8295.01
TCCGCCT400.0295.01
GTACAGT108.4493763E-4295.0295
TTTACCT400.0295.01
TTAGCCT303.6379788E-12295.01
GAGTCGG108.4493763E-4295.0295
CCCTAGG108.4493763E-4295.04
TAGACCT350.0295.01
GGTCCTA1150.0295.02
CGACCTA1150.0295.02
AGGACCT500.0295.01
CGCGCCT255.2568794E-10295.01
GGATCCT157.2136154E-6295.01
CGACCCT206.146911E-8295.01
GCGCCTA1100.0295.02
Produced by FastQC (version 0.11.5)