FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83392
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1110.1331062931696086No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1020.12231389102072142No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1020.12231389102072142No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC990.11871642363775901No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC990.11871642363775901No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC990.11871642363775901No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC970.11631811204911742No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.11511895625479662No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.11391980046047583No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.11391980046047583No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.11391980046047583No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11272064466615504No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11272064466615504No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.11032233307751342No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC910.10912317728319262No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.10792402148887184No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.10672486569455104No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.10672486569455104No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.10552570990023025No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.10552570990023025No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.10432655410590945No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.10432655410590945No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.10432655410590945No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10312739831158863No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10312739831158863No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.10192824251726784No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10072908672294704No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10072908672294704No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10072908672294704No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10072908672294704No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10072908672294704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)