FastQCFastQC Report
Wed 8 May 2024
EGAF00005491435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78000
Total Bases23.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1540.19743589743589746No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1470.18846153846153846No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1460.18717948717948718No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1400.1794871794871795No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1350.17307692307692307No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1330.17051282051282052No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1330.17051282051282052No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1310.16794871794871796No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.16538461538461538No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.16538461538461538No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1260.16153846153846152No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1260.16153846153846152No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1200.15384615384615385No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.15256410256410258No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.15256410256410258No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.15256410256410258No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1180.15128205128205127No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1180.15128205128205127No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.15No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1160.14871794871794872No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.14743589743589744No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.14615384615384616No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.14487179487179488No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.14487179487179488No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.1435897435897436No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.1435897435897436No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14230769230769233No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14230769230769233No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14230769230769233No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14230769230769233No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14230769230769233No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.14230769230769233No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1100.14102564102564102No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1090.13974358974358975No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1090.13974358974358975No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1090.13974358974358975No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.13846153846153844No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.13846153846153844No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1080.13846153846153844No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.13717948717948716No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.13717948717948716No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1070.13717948717948716No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.1358974358974359No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.1358974358974359No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1060.1358974358974359No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.1346153846153846No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1050.1346153846153846No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.13333333333333333No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.13333333333333333No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.13333333333333333No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.13333333333333333No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1040.13333333333333333No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.13205128205128205No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.13205128205128205No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.13205128205128205No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1030.13205128205128205No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13076923076923078No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13076923076923078No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13076923076923078No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1294871794871795No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1294871794871795No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1294871794871795No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1294871794871795No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1294871794871795No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.1282051282051282No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.1282051282051282No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1000.1282051282051282No Hit
GTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.12692307692307692No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.12692307692307692No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.12692307692307692No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.12692307692307692No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.12692307692307692No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12564102564102564No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12564102564102564No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12564102564102564No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12564102564102564No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12564102564102564No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.12564102564102564No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12435897435897436No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.12307692307692308No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC960.12307692307692308No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1217948717948718No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1217948717948718No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1217948717948718No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.1217948717948718No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12051282051282051No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC940.12051282051282051No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.11923076923076924No Hit
GGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.11923076923076924No Hit
ACTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC930.11923076923076924No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11794871794871796No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11794871794871796No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11794871794871796No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11794871794871796No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11794871794871796No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC920.11794871794871796No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11666666666666668No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11666666666666668No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.11666666666666668No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11538461538461539No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11538461538461539No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11538461538461539No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC900.11538461538461539No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11282051282051281No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11282051282051281No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11282051282051281No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11282051282051281No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11282051282051281No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11282051282051281No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11153846153846153No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11153846153846153No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11153846153846153No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11153846153846153No Hit
AAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC870.11153846153846153No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
CTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.11025641025641025No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10897435897435898No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10897435897435898No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10897435897435898No Hit
AATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10897435897435898No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10769230769230768No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10769230769230768No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10769230769230768No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10769230769230768No Hit
ACGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.10769230769230768No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1064102564102564No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1064102564102564No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1064102564102564No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.1064102564102564No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10512820512820513No Hit
ATTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10512820512820513No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC820.10512820512820513No Hit
TGCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
AACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
CTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
GCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10384615384615384No Hit
TAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10256410256410256No Hit
GACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10256410256410256No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10256410256410256No Hit
AGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
TGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
CGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
AGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
GGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.10128205128205128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)