FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69511
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.15105522866884377No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.14817798621800865No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.14530074376717353No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.14242350131633844No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.14098488009092086No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.14098488009092086No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.13954625886550331No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.13810763764008574No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.13666901641466817No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.13379177396383307No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC900.12947591028758038No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.12803728906216283No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.12659866783674528No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.1251600466113277No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.12372142538591015No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.12372142538591015No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.12228280416049259No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.12084418293507501No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.12084418293507501No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.12084418293507501No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.11940556170965747No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.11940556170965747No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11796694048423992No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11796694048423992No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11796694048423992No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11796694048423992No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11796694048423992No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.11796694048423992No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11796694048423992No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.11652831925882234No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.11652831925882234No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.11508969803340478No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.11508969803340478No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11365107680798724No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11365107680798724No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11365107680798724No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11365107680798724No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11365107680798724No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.11365107680798724No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.11221245558256966No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.11221245558256966No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.11221245558256966No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.11221245558256966No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.11221245558256966No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.11221245558256966No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.11221245558256966No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11077383435715212No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11077383435715212No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.11077383435715212No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.11077383435715212No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.11077383435715212No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10933521313173454No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC760.10933521313173454No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10933521313173454No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10933521313173454No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC760.10933521313173454No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10933521313173454No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC760.10933521313173454No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10789659190631698No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10789659190631698No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10789659190631698No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10789659190631698No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10789659190631698No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.10645797068089943No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10645797068089943No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10645797068089943No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10645797068089943No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10645797068089943No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10645797068089943No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.10501934945548186No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10501934945548186No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.10358072823006431No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.10358072823006431No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10358072823006431No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.10358072823006431No Hit
GATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10358072823006431No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.10358072823006431No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10358072823006431No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10214210700464675No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10214210700464675No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10214210700464675No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10070348577922918No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10070348577922918No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10070348577922918No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10070348577922918No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10070348577922918No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10070348577922918No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10070348577922918No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10070348577922918No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.10070348577922918No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)