FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66375
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCTA1250.0295.000032
CGTCCTA1250.0295.000032
AGCCTAC3400.0295.03
AATCCTA950.0295.02
CACACCT255.2568794E-10295.01
CTCCCTA900.0295.02
AAATCCT255.2568794E-10295.01
GTCGCCT350.0295.01
CATCCTA1700.0295.02
GGTCCCT255.2568794E-10295.01
CTCTCCT255.2568794E-10295.01
ACACCTA950.0295.02
CGGACCT255.2568794E-10295.01
GAGTCCT255.2568794E-10295.01
TTCCCTA1000.0295.02
GACACCT255.2568794E-10295.01
TTCCCCT255.2568794E-10295.01
TGCCCCT255.2568794E-10295.01
TCAGCCT350.0295.01
CACCCTA900.0295.02
Produced by FastQC (version 0.11.5)