FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74380
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC780.10486689970422157No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10083355740790535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCCT350.0294.995331
CTTCCCT350.0294.995331
CCGGCCT350.0294.995331
CGTTCCT350.0294.995331
GGAACCT350.0294.995331
GTTCCCT350.0294.995331
CACCCTA1400.0294.995332
TGCACCT350.0294.995331
TGATCCT255.2568794E-10294.99531
GCCCCTA1050.0294.99532
ATCTCCT255.2568794E-10294.99531
TGCCCCT255.2568794E-10294.99531
AGATCCT255.2568794E-10294.99531
ACGCCTA500.0294.99532
CAGCCTA750.0294.99532
AGCCCTA900.0294.99532
GAGTCCT255.2568794E-10294.99531
GGGTCCT450.0294.99531
AAGACCT500.0294.99531
TGAACCT255.2568794E-10294.99531
Produced by FastQC (version 0.11.5)