FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85828
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1010.1176772148949061No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC1000.11651209395535256No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC990.11534697301579903No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC990.11534697301579903No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC980.11418185207624552No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC980.11418185207624552No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC950.11068648925758494No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC940.1095213683180314No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC930.10835624737847789No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC930.10835624737847789No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC910.10602600549937083No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC910.10602600549937083No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC910.10602600549937083No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.10253064268071026No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.10136552174115673No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.10136552174115673No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC860.1002004008016032No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC860.1002004008016032No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.1002004008016032No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC860.1002004008016032No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCT550.0295.000031
CCCTCCT550.0295.000031
GCACCTA1750.0295.000032
TTACCTA1750.0295.000032
CACCCTA1100.0295.000032
CGCCCTA1100.0295.000032
GCTCCTA1100.0295.000032
TCGCCTA950.0295.02
GTTGATT350.0295.0295
TCTGCCT303.6379788E-12295.01
TTGGCCT303.6379788E-12295.01
GTCGCCT157.2170824E-6295.01
AGATCCT450.0295.01
CGAACCT303.6379788E-12295.01
ACGCCTA1000.0295.02
GTTTCCT350.0295.01
CAACGGG108.4514054E-4295.06
CAGCCTA1150.0295.02
GAGTCCT157.2170824E-6295.01
TAGACCT450.0295.01
Produced by FastQC (version 0.11.5)