FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48118
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT490.10183299389002036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT350.0295.000031
AGTACCT350.0295.000031
GTGCCTA350.0295.000032
TGATCCT157.197401E-6295.01
TCGCCCT255.2386895E-10295.01
GTCGCCT108.439877E-4295.01
CGAACCT108.439877E-4295.01
GACACCT108.439877E-4295.01
GATCCTA550.0295.02
ATAGCCT303.6379788E-12295.01
GCGCCTA650.0295.02
TAGCCTA800.0295.02
CGTTCCT255.2386895E-10295.01
ACCCTAC3600.0295.03
TACGCCT206.12381E-8295.01
CGCTCCT303.6379788E-12295.01
TGCGCCT157.197401E-6295.01
TCGCTTT108.439877E-4295.0295
AACGCCT157.197401E-6295.01
CATACCT206.12381E-8295.01
Produced by FastQC (version 0.11.5)