FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47555
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.17243192093365575No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.155609294501104No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.153506466197035No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.15140363789296604No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.14719798128482808No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.14719798128482808No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.14719798128482808No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.14509515298075912No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.14509515298075912No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.14299232467669015No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.1408894963726212No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.1387866680685522No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.1387866680685522No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.1387866680685522No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.13668383976448323No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.13668383976448323No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.13458101146041426No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.13458101146041426No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.13458101146041426No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1303753548522763No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1303753548522763No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1303753548522763No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1303753548522763No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.1303753548522763No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.12616969824413837No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.12616969824413837No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.12616969824413837No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.12616969824413837No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.1240668699400694No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.1240668699400694No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.1240668699400694No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.12196404163600041No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.12196404163600041No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.12196404163600041No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.12196404163600041No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.12196404163600041No Hit
GGGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.11986121333193145No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.11986121333193145No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.11986121333193145No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.11986121333193145No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.11986121333193145No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.11775838502786247No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.11775838502786247No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.11775838502786247No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.11775838502786247No Hit
CAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.11775838502786247No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.11775838502786247No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.1156555567237935No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.1156555567237935No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.1156555567237935No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.1156555567237935No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.1156555567237935No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC550.1156555567237935No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC540.11355272841972452No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC540.11355272841972452No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC540.11355272841972452No Hit
GCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11144990011565557No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11144990011565557No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11144990011565557No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11144990011565557No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11144990011565557No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.11144990011565557No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.10934707181158658No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.10934707181158658No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.10934707181158658No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.10934707181158658No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC520.10934707181158658No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC510.1072442435075176No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC510.1072442435075176No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC510.1072442435075176No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC510.1072442435075176No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC510.1072442435075176No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
CTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
TAGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC500.10514141520344865No Hit
TACGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
AGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
GAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
AAGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC490.10303858689937966No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.1009357585953107No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.1009357585953107No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.1009357585953107No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.1009357585953107No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.1009357585953107No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC480.1009357585953107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)