FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47555
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10622.233203658921249No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10372.1806329513195246No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9872.075491536116076No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9802.0607717379875936No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9501.9976868888655241No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8491.7853012301545579No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7531.5834297129639365No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6971.465671327936074No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5591.1754810219745557No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5541.1649668804542108No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5281.1102933445484175No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5251.1039848596362107No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5071.0661339501629692No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5031.0577226369466932No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4991.0493113237304175No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4891.0282830406897276No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4771.0030491010409002No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4110.8642624329723477No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3920.8243086951950372No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3560.7486068762485543No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3460.7275785932078646No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3400.7149616233834507No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3110.6539796025654505No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3100.6518767742613816No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3090.6497739459573126No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3030.6371569761328988No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2740.5761749553148985No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2700.5677636420986226No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2550.536221217537588No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2520.5299127326253811No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2510.5278099043213123No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2440.5130901061928294No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2320.48785616654400166No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2180.45841657028703603No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.45000525707076017No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2130.4479024287666912No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2120.4457996004626223No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2050.4310798023341394No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1970.41425717590158767No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1950.41005151929344974No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1920.4037430343812428No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1910.4016402060771738No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1900.3995373777731048No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1890.3974345494690359No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1870.39322889286089785No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1850.38902323625276No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.37430343812427713No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1760.3700977815161392No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1750.3679949532120702No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1720.36168646829986334No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1700.35748081169172535No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1690.3553779833876564No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1640.3448638418673115No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1600.33645252865103564No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1520.31962990221848386No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1520.31962990221848386No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1490.31332141730627694No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1480.311218589002208No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1410.29649879087372516No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1340.2817789927452424No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1310.27547050783303545No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1290.27126485122489746No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1260.26495636631269054No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1260.26495636631269054No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1250.2628535380086216No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1210.2544422247923457No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1160.24392808327200083No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1040.21869414362317316No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1030.21659131531910422No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1030.21659131531910422No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1030.21659131531910422No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.2144884870150352No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1010.21238565871096624No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1010.21238565871096624No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA990.20818000210282833No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA970.20397434549469037No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA970.20397434549469037No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC970.20397434549469037No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.2018715171906214No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA960.2018715171906214No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA950.1997686888865524No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA910.19135737567027652No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA910.19135737567027652No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA910.19135737567027652No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA910.19135737567027652No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG900.18925454736620756No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA900.18925454736620756No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG900.18925454736620756No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA890.18715171906213857No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA880.1850488907580696No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG880.1850488907580696No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA880.1850488907580696No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG860.18084323414993167No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA860.18084323414993167No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.17874040584586268No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA840.1766375775417937No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.17453474923772475No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA830.17453474923772475No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG830.17453474923772475No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA830.17453474923772475No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA810.17032909262958681No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA800.16822626432551782No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA800.16822626432551782No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA780.1640206077173799No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.1640206077173799No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG770.1619177794133109No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.15981495110924193No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA750.15771212280517297No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA750.15771212280517297No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA750.15771212280517297No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG740.155609294501104No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA740.155609294501104No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA730.153506466197035No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA710.14930080958889708No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA700.14719798128482808No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA690.14509515298075912No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA690.14509515298075912No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA690.14509515298075912No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA690.14509515298075912No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA670.1408894963726212No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA660.1387866680685522No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA660.1387866680685522No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA650.13668383976448323No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA650.13668383976448323No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA650.13668383976448323No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA630.13247818315634527No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA630.13247818315634527No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.1303753548522763No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA610.12827252654820734No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA580.12196404163600041No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA580.12196404163600041No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA580.12196404163600041No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA580.12196404163600041No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.12196404163600041No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA580.12196404163600041No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA570.11986121333193145No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA570.11986121333193145No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA570.11986121333193145No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA560.11775838502786247No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA560.11775838502786247No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA560.11775838502786247No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA560.11775838502786247No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA550.1156555567237935No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA530.11144990011565557No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA520.10934707181158658No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA500.10514141520344865No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA490.10303858689937966No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA490.10303858689937966No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA490.10303858689937966No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA480.1009357585953107No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG480.1009357585953107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCC700.0295.0295
CGTTATC108.4395724E-4295.0295
CGTTAGC550.0295.0295
TGTTAGC108.4395724E-4295.0295
ACTCGGC108.4395724E-4295.06
TAGATCC108.4395724E-4295.0295
GGTTAGC108.4395724E-4295.0295
TGACTAC255.2386895E-10295.01
GGACTAC44400.0294.66781
TACTCGG8200.0293.20125
ACTCGGG8100.0293.179026
GACTACT19950.0292.04262
TCGGGTT4500.0291.72228
ACCCGGG3700.0291.013526
ACGGGTA3650.0290.958928
CACGGGT6750.0290.629647
ACGGGTT3150.0290.317478
TACAAGG8150.0289.57065
CTACCGG2550.0289.21574
CTACTAG7500.0289.14
Produced by FastQC (version 0.11.5)