FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60968
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA700.0295.000032
ACGCCCT350.0295.000031
AAGCCTA700.0295.000032
ACGTCCT350.0295.000031
CGCCCTA950.0295.000032
TCGCCCT157.2068397E-6295.01
AGATCCT157.2068397E-6295.01
CGAACCT303.6379788E-12295.01
CGTGCCT108.445407E-4295.01
TAGACCT500.0295.01
GTCTCCT206.13727E-8295.01
CGACCCT157.2068397E-6295.01
GCGCCTA1050.0295.02
GCGCCCT108.445407E-4295.01
GTCTACG157.2068397E-6295.03
TAGCCTA1100.0295.02
GACCCTA1000.0295.02
CGTTCCT255.2568794E-10295.01
CCGTCCT206.13727E-8295.01
ACCTCCT157.2068397E-6295.01
Produced by FastQC (version 0.11.5)