FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60326
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.1541623843782117No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.14753174418990153No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.14753174418990153No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.14421642409574645No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.14421642409574645No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.14090110400159134No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.14090110400159134No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13758578390743625No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.13758578390743625No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.1359281238603587No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.1359281238603587No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.1359281238603587No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.13427046381328117No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.13261280376620363No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.13261280376620363No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.13261280376620363No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1309551437191261No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1309551437191261No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12763982362497098No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12763982362497098No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12763982362497098No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12763982362497098No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12763982362497098No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.12763982362497098No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.12598216357789344No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.12598216357789344No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.12598216357789344No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.1243245035308159No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.1243245035308159No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.12266684348373835No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.12266684348373835No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.12266684348373835No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.12266684348373835No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.12266684348373835No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.12100918343666081No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12100918343666081No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.12100918343666081No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12100918343666081No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12100918343666081No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.12100918343666081No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11935152338958327No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11935152338958327No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11935152338958327No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11935152338958327No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11935152338958327No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11935152338958327No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11935152338958327No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.11935152338958327No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11769386334250571No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11769386334250571No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11769386334250571No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11769386334250571No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11769386334250571No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11603620329542817No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11603620329542817No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11603620329542817No Hit
TCGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11603620329542817No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11603620329542817No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11603620329542817No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.11437854324835063No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.11437854324835063No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.11437854324835063No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11437854324835063No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.11437854324835063No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11437854324835063No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.11437854324835063No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11272088320127309No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11272088320127309No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.11272088320127309No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.11272088320127309No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.11106322315419553No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.11106322315419553No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11106322315419553No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11106322315419553No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11106322315419553No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.11106322315419553No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.11106322315419553No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.11106322315419553No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.11106322315419553No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10940556310711799No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10940556310711799No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10940556310711799No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10774790306004045No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10774790306004045No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10774790306004045No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10774790306004045No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC650.10774790306004045No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10609024301296291No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10609024301296291No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10609024301296291No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10609024301296291No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10609024301296291No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10609024301296291No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10609024301296291No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC640.10609024301296291No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.10443258296588535No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10443258296588535No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.10443258296588535No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.10443258296588535No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10443258296588535No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10443258296588535No Hit
TGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.10443258296588535No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10443258296588535No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.10443258296588535No Hit
ACCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10443258296588535No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.10443258296588535No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC620.10277492291880781No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC620.10277492291880781No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC620.10277492291880781No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.10277492291880781No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10111726287173027No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC610.10111726287173027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTA550.0295.000032
GGACCTA950.0295.000032
TGATCCT350.0295.01
GCCCCTA850.0295.02
GTTTGTC255.2568794E-10295.0295
AGTCCTA750.0295.02
GTCGCCT255.2568794E-10295.01
AAATCCT350.0295.01
TGCCCCT303.6379788E-12295.01
CGAACCT206.1367246E-8295.01
ACGCCTA900.0295.02
CCCTCCT400.0295.01
TCACCCT157.2064577E-6295.01
CACGGGA157.2064577E-6295.06
AGCCCTA400.0295.02
CAGCCCT108.445184E-4295.01
GGGTCCT206.1367246E-8295.01
GATCCCT157.2064577E-6295.01
GTCTCCT255.2568794E-10295.01
GGTCCCT206.1367246E-8295.01
Produced by FastQC (version 0.11.5)