FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60326
Sequences flagged as poor quality0
Sequence length301
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA12742.111858899976793No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA12742.111858899976793No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA10471.73557006929019No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA10221.6941285681132512No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA9961.6510294068892353No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA9681.604614925571064No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA9631.5963266253356763No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA9351.5499121440175048No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA9221.528362563405497No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA8781.455425521334085No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8691.440506580910387No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8141.3493352783211219No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA8041.3327586778503464No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7431.2316414149786161No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7251.2018035341312203No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6971.155389052813049No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6881.1404701123893513No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6781.1238935119185758No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6431.0658754102708616No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6391.0592447700825516No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6321.0476411497530087No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6221.0310645492822332No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6171.0227762490468455No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6141.017803268905613No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5820.9647581473991313No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5660.9382355866458907No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5600.9282896263634254No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5530.9166860060338825No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5250.8702715247157112No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5150.8536949242449358No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4710.7807578821735239No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA4640.7691542618439811No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4530.750920001326128No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4180.692901899678414No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3830.6348837980306998No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3800.6299108178894672No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3780.6265954977953121No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3660.6067035772303816No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3480.5768656963829858No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3430.5685773961475981No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3380.5602890959122103No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3380.5602890959122103No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2910.4823790736995657No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2910.4823790736995657No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2580.4276762921460067No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2540.4210456519576965No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2500.41441501176938633No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2460.40778437158107617No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2440.4044690514869211No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2400.3978384112986109No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2280.37794649073368036No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2260.3746311706395253No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2230.3696581904982926No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2230.3696581904982926No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2150.3563969101216723No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2150.3563969101216723No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2120.35142392998043964No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2090.346450949839207No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1990.3298743493684315No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1770.2934058283327255No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1750.2900905082385704No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.2900905082385704No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1740.2884328481914929No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1710.28345986805026024No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1700.2818022080031827No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1690.28014454795610516No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1690.28014454795610516No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1630.27019858767363986No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1610.2668832675794848No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1590.2635679474853297No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1560.2585949673440971No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.2569373072970195No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.25030666710870936No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1500.2486490070616318No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1490.24699134701455425No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1450.24036070682624408No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1410.2337300666379339No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1400.23207240659085634No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1370.22709942644962372No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1350.2237841063554686No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.2237841063554686No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1340.22212644630839107No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1320.21881112621423598No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.2154958061200809No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1290.21383814607300336No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1240.20554984583761562No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.20554984583761562No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1230.20389218579053808No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1220.20223452574346054No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1200.19891920564930546No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1180.19560388555515035No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1120.1856579252726851No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1120.1856579252726851No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1120.1856579252726851No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1110.18400026522560753No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1110.18400026522560753No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1090.18068494513145245No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1090.18068494513145245No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1060.17571196499021982No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1060.17571196499021982No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1060.17571196499021982No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1050.17405430494314228No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1050.17405430494314228No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1050.17405430494314228No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1050.17405430494314228No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1040.17239664489606474No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1030.17073898484898717No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.16908132480190963No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1000.16576600470775452No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1000.16576600470775452No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA990.16410834466067697No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA980.16245068461359943No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA980.16245068461359943No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT950.1574777044723668No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA930.1541623843782117No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA930.1541623843782117No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT930.1541623843782117No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA920.15250472433113416No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA910.15084706428405661No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA900.14918940423697907No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA900.14918940423697907No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA890.14753174418990153No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA890.14753174418990153No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA890.14753174418990153No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA870.14421642409574645No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT860.1425587640486689No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT850.14090110400159134No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA840.1392434439545138No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA810.13427046381328117No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA810.13427046381328117No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA790.1309551437191261No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA780.12929748367204855No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA750.1243245035308159No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA750.1243245035308159No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT730.12100918343666081No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA720.11935152338958327No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA720.11935152338958327No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA710.11769386334250571No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA700.11603620329542817No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA700.11603620329542817No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT690.11437854324835063No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT690.11437854324835063No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA690.11437854324835063No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT690.11437854324835063No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA670.11106322315419553No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA670.11106322315419553No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA670.11106322315419553No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA630.10443258296588535No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA620.10277492291880781No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA610.10111726287173027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG6000.0294.998356
TCGGGTT1700.0294.998358
CGTTATC108.4453257E-4294.99835295
CTCGGGT4500.0294.998357
CTACGGG108.4453257E-4294.998354
AATTCGC108.4453257E-4294.99835295
AGTTAGG108.4453257E-4294.99835295
CTACAAG8700.0294.998354
CGTTAGC600.0294.99832295
AGTTGGC157.206623E-6294.99832295
TACTGGG4750.0294.998325
TACACGG6050.0294.998325
ACTGGGG4750.0294.998326
GGACTAC53550.0294.660681
GACTACA21450.0294.31072
CAAGGGT8750.0293.312627
ACAAGGG8750.0293.312626
TACAAGG8750.0293.312625
CTACTAG5050.0292.077584
TACTAGG5050.0292.077585
Produced by FastQC (version 0.11.5)