FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70666
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.12452947669317634No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11179350748591968No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.11179350748591968No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10754818441683413No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10754818441683413No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10754818441683413No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10754818441683413No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10754818441683413No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10613307672713893No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10613307672713893No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10471796903744376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCT550.0295.000031
TAGCCTA1100.0295.000032
CTCTCCT550.0295.000031
GCCCTAG108.4482506E-4295.03
CTACAGG108.4482506E-4295.06
ATCCCCT255.2568794E-10295.01
AGCCTAC3500.0295.03
ACGCCTA1350.0295.02
CCTACGA108.4482506E-4295.05
CCTACCT255.2568794E-10295.01
TGACCTC108.4482506E-4295.02
TTCGCCT400.0295.01
TTACCCT206.1441824E-8295.01
CGCCTAC3700.0295.03
ACGTCCT255.2568794E-10295.01
AGGACCT450.0295.01
GTCGCCT255.2568794E-10295.01
GGTCCTA800.0295.02
GGCGCCT206.1441824E-8295.01
GGACCCT400.0295.01
Produced by FastQC (version 0.11.5)