FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68800
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.12645348837209303No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.12209302325581395No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.11918604651162791No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11337209302325581No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.11337209302325581No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.1119186046511628No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.1119186046511628No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.1119186046511628No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11046511627906977No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.11046511627906977No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.10901162790697674No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.10755813953488372No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.10610465116279069No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.10610465116279069No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.10610465116279069No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10465116279069768No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.10465116279069768No Hit
CTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.10319767441860465No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1002906976744186No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1002906976744186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCT550.0295.000031
TTGCCTA550.0295.000032
GCCCCTA700.0295.02
ACGCCTA500.0295.02
GATCCTA900.0295.02
TTGTCCT400.0295.01
CAACCTA1350.0295.02
GTCTCCT255.2568794E-10295.01
TCCCTAC3350.0295.03
CGCGCCT255.2568794E-10295.01
CGTTCCT255.2568794E-10295.01
GCACCCT108.447771E-4295.01
AGTGCCT108.447771E-4295.01
TGACCTA1350.0295.02
TACGCCT206.142909E-8295.01
TACGAGA206.142909E-8295.07
ATCCCCT206.142909E-8295.01
TATTATC108.447771E-4295.0295
AGTTCCT255.2568794E-10295.01
GGCGCCT108.447771E-4295.01
Produced by FastQC (version 0.11.5)