FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82005
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA1250.0295.000032
GCACCCT550.0295.000031
CGGTCCT350.0295.000031
TAGTCCT350.0295.000031
TGATCCT400.0295.01
ATCCTCG108.4507227E-4295.03
CTGTCCT500.0295.01
GTCCTAG108.4507227E-4295.03
GGTCCTA1450.0295.02
TTGTCCT400.0295.01
GCGCCCT206.150185E-8295.01
TTCCCTA1350.0295.02
GTAGCCT157.2159182E-6295.01
TACGCGA108.4507227E-4295.07
TACGCCT206.150185E-8295.01
GTTCCCT400.0295.01
GGGCCTA1000.0295.02
GGACCTA1150.0295.02
GGCGCCT450.0295.01
GATTCCT255.275069E-10295.01
Produced by FastQC (version 0.11.5)