FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55606
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC920.1654497716073805No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC900.16185303744200266No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC890.16005467035931376No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC880.15825630327662482No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC860.15465956911124698No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC860.15465956911124698No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.15286120202855807No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC850.15286120202855807No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.15106283494586917No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.15106283494586917No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.15106283494586917No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.15106283494586917No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC840.15106283494586917No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC830.14926446786318023No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.1474661007804913No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC820.1474661007804913No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.14386936661511346No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.14386936661511346No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.14386936661511346No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.14386936661511346No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC800.14386936661511346No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.14207099953242455No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC790.14207099953242455No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.1384742653670467No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.1384742653670467No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.1384742653670467No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC770.1384742653670467No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.1366758982843578No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.1366758982843578No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.1366758982843578No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.1366758982843578No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC760.1366758982843578No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC750.1348775312016689No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.13307916411897996No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.13307916411897996No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.13307916411897996No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.13307916411897996No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC740.13307916411897996No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.13128079703629106No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.13128079703629106No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.13128079703629106No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC730.13128079703629106No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
AAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC720.12948242995360215No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.12768406287091322No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.12768406287091322No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.12768406287091322No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.12768406287091322No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.12768406287091322No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC710.12768406287091322No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC700.12588569578822428No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC700.12588569578822428No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC700.12588569578822428No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.12408732870553536No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.12408732870553536No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.12408732870553536No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.12408732870553536No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.12408732870553536No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC690.12408732870553536No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC680.12228896162284646No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.12049059454015754No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.12049059454015754No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.12049059454015754No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.12049059454015754No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC670.12049059454015754No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.11869222745746862No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.11869222745746862No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC660.11869222745746862No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1168938603747797No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1168938603747797No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1168938603747797No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.1168938603747797No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1168938603747797No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC650.1168938603747797No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.11509549329209079No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.11509549329209079No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.11509549329209079No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC640.11509549329209079No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC630.11329712620940185No Hit
GTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC630.11329712620940185No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC630.11329712620940185No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
ACGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC620.11149875912671295No Hit
CCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC620.11149875912671295No Hit
ACTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC610.10970039204402403No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC610.10970039204402403No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC610.10970039204402403No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC610.10970039204402403No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10790202496133511No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC600.10790202496133511No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC600.10790202496133511No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10610365787864619No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC590.10610365787864619No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10610365787864619No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC590.10610365787864619No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC590.10610365787864619No Hit
TGGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC590.10610365787864619No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
GTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
TCTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC580.10430529079595728No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC580.10430529079595728No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC570.10250692371326835No Hit
GCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC570.10250692371326835No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC560.10070855663057944No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCC560.10070855663057944No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC560.10070855663057944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCT206.105074E-8295.263731
CGTTCCT206.105074E-8295.263731
ACAGCCT108.420828E-4295.263731
TTGTCCT350.0295.263731
TGGTCCT157.177756E-6295.263731
ACGCCTA500.0295.263732
TTCACCT450.0295.263731
TGCACCT157.177756E-6295.263731
CCAACCT303.6379788E-12295.263731
GTGTCCT206.105074E-8295.263731
ATCTCCT108.420828E-4295.263731
CACACCT400.0295.263731
TCGACCT303.6379788E-12295.263731
CTCCCTA650.0295.263732
AAATCCT303.6379788E-12295.263731
TTCGCCT108.420828E-4295.263731
TTACCCT255.2204996E-10295.263731
GGACCTA750.0295.263732
GCGACCT206.105074E-8295.263731
CAACCTA1200.0295.263732
Produced by FastQC (version 0.11.5)