FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78157
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.12027073710608135No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.11771178525276046No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.11643230932610003No Hit
ATCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.11515283339943959No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT890.11387335747277914No Hit
CTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT880.1125938815461187No Hit
GTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.11131440561945828No Hit
CTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.11003492969279784No Hit
TCCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.11003492969279784No Hit
TAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.11003492969279784No Hit
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT860.11003492969279784No Hit
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10875545376613739No Hit
ACCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10875545376613739No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10875545376613739No Hit
ACTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.10875545376613739No Hit
CCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.10619650191281652No Hit
AAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.10619650191281652No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.10491702598615607No Hit
TTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.10491702598615607No Hit
ATTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.10491702598615607No Hit
ATAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT810.10363755005949563No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.10235807413283518No Hit
CTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.10107859820617474No Hit
TGCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.10107859820617474No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.10107859820617474No Hit
CTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT790.10107859820617474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA400.0295.02
GCCCCTA1200.0295.02
GTCGCCT206.148366E-8295.01
CCCCTAG108.449965E-4295.03
AGATCCT157.2146213E-6295.01
GGTCCTA1600.0295.02
GGATCCT350.0295.01
GTTACCT255.275069E-10295.01
TTCCCTA1200.0295.02
TACGCCT255.275069E-10295.01
TCTACGG157.2146213E-6295.04
TCCACCT303.6379788E-12295.01
TTGCCCT157.2146213E-6295.01
CGGTAGG108.449965E-4295.09
CGATCCT255.275069E-10295.01
CCGCCCT255.275069E-10295.01
CCCGCCT206.148366E-8295.01
CGGACCT255.275069E-10295.01
GCCTACG11700.0293.739324
TCCTACG20450.0291.393654
Produced by FastQC (version 0.11.5)