FastQCFastQC Report
Wed 8 May 2024
EGAF00005491490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78157
Total Bases23.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC24543.1398339240247193No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC20182.5819824200007675No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC20072.567908184807503No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC18042.308174571695434No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC17972.2992182402088104No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC16512.1124147549163865No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11451.4649999360262036No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10281.3153012526069323No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9471.2116637025474366No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9421.2052663229141343No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9181.1745589006742838No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC7901.0107859820617475No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7590.971122228335274No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7580.9698427524086135No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC7580.9698427524086135No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6740.8623667745691365No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6650.8508514912291926No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6330.8099082615760583No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5930.7587292245096409No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5710.7305807541231112No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5670.7254628504164694No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5190.6640480059367684No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4260.5450567447573473No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3770.48236242435098586No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3770.48236242435098586No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3720.47596504471768364No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3710.4746855687910232No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3530.45165500211113524No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3460.44269867062451224No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3460.44269867062451224No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3430.4388602428445309No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3410.43630129099121No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3370.43118338728456823No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3250.41582967616464295No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3190.4081528206046803No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3140.40175544097137816No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3070.39279910948475505No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2950.37744539836482977No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2900.37104801873152754No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2850.36465063909822537No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2850.36465063909822537No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2790.3569737835382627No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2760.3531353557582814No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2720.34801745205163964No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2680.3428995483449979No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2660.340340596491677No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2600.3326637409317144No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2560.32754583722507263No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2550.3262663612984122No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2260.28916155942525945No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2230.2853231316452781No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2200.2814847038652968No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1960.2507772816254462No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1930.24693885384546488No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1860.23798252235884182No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1820.23286461865220007No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.22134933531225612No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1710.21879038345893523No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.19959824455902864No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1440.18424453343910333No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1430.1829650575124429No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1410.18040610565912202No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1400.17912662973246157No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1390.17784715380580113No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1330.1701702982458385No Hit
GGACTACTGGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1310.16761134639251762No Hit
GGACTACTGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1300.1663318704658572No Hit
GGACTACTGGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1280.16377291861253632No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1270.16249344268587587No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1230.1573755389792341No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1220.15609606305257365No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1220.15609606305257365No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1200.15353711119925278No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1190.15225763527259234No Hit
GGACTACTCGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1180.1509781593459319No Hit
GGACTACTGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1180.1509781593459319No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1180.1509781593459319No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.14969868341927148No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1160.14841920749261103No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1140.14586025563929014No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1130.14458077971262973No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1100.1407423519326484No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1100.1407423519326484No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1100.1407423519326484No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1090.13946287600598795No Hit
GGACTACTAGGGTTTCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA1090.13946287600598795No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1090.13946287600598795No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1090.13946287600598795No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1080.1381834000793275No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1050.13434497229934617No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1030.1317860204460253No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1020.13050654451936486No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1010.1292270685927044No Hit
GGACTACTCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1000.127947592666044No Hit
GGACTACTGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG990.12666811673938355No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA990.12666811673938355No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA990.12666811673938355No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG990.12666811673938355No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA980.1253886408127231No Hit
GGACTACTCGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA980.1253886408127231No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA980.1253886408127231No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA980.1253886408127231No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA970.12410916488606266No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA960.12282968895940224No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA960.12282968895940224No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC950.1215502130327418No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC950.1215502130327418No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA940.12027073710608135No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC940.12027073710608135No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA940.12027073710608135No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA940.12027073710608135No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA930.11899126117942091No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA920.11771178525276046No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG910.11643230932610003No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA890.11387335747277914No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.11387335747277914No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA890.11387335747277914No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC880.1125938815461187No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC870.11131440561945828No Hit
GGACTACTAGGGTATCTAAAAGGCTAGATAGTAACTGTGGAATTTTGCAA870.11131440561945828No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA860.11003492969279784No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA860.11003492969279784No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA850.10875545376613739No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.10875545376613739No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA840.10747597783947695No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA830.10619650191281652No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA830.10619650191281652No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT820.10491702598615607No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA820.10491702598615607No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC790.10107859820617474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)