FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69953
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1350.0295.02
GCCCCTA900.0295.02
ACGCCTA1100.0295.02
CCCTCGG108.448072E-4295.04
GGTAATC108.448072E-4295.0295
CGGCCCT400.0295.01
GGTCCGG108.448072E-4295.0295
CGACCTA1050.0295.02
GCGCCCT350.0295.01
CCTCGGA157.211389E-6295.05
GGCCCTA950.0295.02
TTCCCTA1150.0295.02
ATATCCT500.0295.01
TACGCCT255.2568794E-10295.01
CTCGGAG157.211389E-6295.06
CTACGAG108.448072E-4295.06
CGTACCT157.211389E-6295.01
CTCCTCG108.448072E-4295.03
CTCCTAG157.211389E-6295.03
TTCGCCT350.0295.01
Produced by FastQC (version 0.11.5)