FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69953
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5840.8348462539133418No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5780.8262690663731362No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5360.7662287535916973No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5230.7476448472545852No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4470.6390004717453147No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4360.6232756279216045No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4260.6089803153545953No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3930.5618057838834646No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3920.5603762526267637No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3820.5460809400597544No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3800.5432218775463525No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3710.5303560962360442No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3680.5260675024659415No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3660.5232084399525396No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3600.514631252412334No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3400.4860406272783154No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3140.4488728146040913No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2980.4260003144968765No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2920.4174231269566709No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2920.4174231269566709No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2880.4117050019298672No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2850.40741640815976443No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2770.395980158106157No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2660.38025531428244674No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2620.37453718925564305No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2590.3702485954855403No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2580.36881906422883937No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2570.36738953297213844No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2560.3659600017154375No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2510.35881234543193286No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2470.35309422040512917No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2420.3459465641216245No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2390.34165797035152173No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2380.3402284390948208No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2370.3387989078381199No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2330.33308078281131615No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2320.3316512515546152No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2300.3287921890412134No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2280.32593312652781153No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2240.32021500150100785No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2230.31878547024430687No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2220.31735593898760595No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2200.3144968764742041No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2180.31163781396080226No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2160.3087787514474004No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2130.30449015767729765No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2050.2930539076236902No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2050.2930539076236902No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2040.2916243763669893No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1980.2830471888267837No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1890.27018140751647535No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1860.2658928137463726No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1820.2601746887195689No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1760.2515975011793633No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1730.24730890740926054No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1720.2458793761525596No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1700.2430203136391577No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1700.2430203136391577No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1690.24159078238245676No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1690.24159078238245676No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1660.237302188612354No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1650.23587265735565305No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1610.23015453232884936No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1600.22872500107214844No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1580.22586593855874657No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.22443640730204564No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1500.21442968850513916No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1490.21300015724843824No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.20871156347833544No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1450.20728203222163452No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1450.20728203222163452No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1440.2058525009649336No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1420.20299343845153173No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1410.2015639071948308No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1410.2015639071948308No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1400.20013437593812988No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1380.197275313424728No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1370.1958457821680271No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1350.19298671965462524No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1340.19155718839792432No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1330.19012765714122337No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1320.18869812588452245No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1320.18869812588452245No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1300.1858390633711206No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1300.1858390633711206No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1290.18440953211441968No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1290.18440953211441968No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1280.18298000085771876No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1270.1815504696010178No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1270.1815504696010178No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1270.1815504696010178No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1260.1801209383443169No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1260.1801209383443169No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1240.17726187583091504No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1220.17440281331751317No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1170.16725515703400856No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.1658256257773076No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1150.1643960945206067No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1140.16296656326390577No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1120.16010750075050392No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1110.15867796949380297No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1110.15867796949380297No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.15724843823710205No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1080.1543893757237002No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1050.1501007819535974No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.1486712506968965No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1030.14724171944019557No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1030.14724171944019557No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1020.14581218818349465No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1020.14581218818349465No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1010.1443826569267937No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1010.1443826569267937No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1000.14295312567009277No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1000.14295312567009277No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC990.14152359441339185No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC980.14009406315669093No Hit
GGACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA970.13866453189999No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC940.1343759381298872No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC930.1329464068731863No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA920.13151687561648537No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.13151687561648537No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA910.13008734435978445No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT910.13008734435978445No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC910.13008734435978445No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC910.13008734435978445No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.1286578131030835No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.12579875058968165No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA880.12579875058968165No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA870.12436921933298073No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC860.1229396880762798No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA860.1229396880762798No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT850.12151015681957884No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT850.12151015681957884No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA850.12151015681957884No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA840.12008062556287792No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC840.12008062556287792No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.118651094306177No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA820.11722156304947606No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC820.11722156304947606No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA810.11579203179277514No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA810.11579203179277514No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC810.11579203179277514No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC800.11436250053607422No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.11293296927937328No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC790.11293296927937328No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA790.11293296927937328No Hit
GGACTACAAGGGTATCTAATACCTCTTGTCCATGTCTTATGATACGGCCA770.11007390676597142No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA760.1086443755092705No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA760.1086443755092705No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA760.1086443755092705No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.1086443755092705No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.10721484425256958No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA740.10578531299586864No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC730.10435578173916772No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA720.1029262504824668No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC720.1029262504824668No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA720.1029262504824668No Hit
GGACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA720.1029262504824668No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA720.1029262504824668No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC710.10149671922576586No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA700.10006718796906494No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA700.10006718796906494No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA700.10006718796906494No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG108.448072E-4295.0295
CGGGGGA108.448072E-4295.08
ATTTCGC108.448072E-4295.0295
GGACTAC67050.0293.680081
CTACCCG4800.0291.92714
TACTCGG7900.0291.265845
CGGGTAT13000.0290.461559
TCGGGTT3200.0290.390628
CCGGGTA3100.0290.241948
AAGGGTA8450.0289.763348
TACACGG8350.0289.700625
ACAAGGG13650.0289.597086
CTACTCG7950.0289.433964
CTCGGGT7950.0289.433967
TACTAGG10550.0289.40765
ACGGGTA5200.0289.326938
GACTACC15250.0289.196752
ACCCGGG4850.0288.917546
CAAGGGT13650.0288.516487
TACTGGG6650.0288.345865
Produced by FastQC (version 0.11.5)