FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76686
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA27883.6356049344078447No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA21442.795816707091255No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA17992.345930156743082No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA16002.08643037842631No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA15782.0577419607229483No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA15642.039485694911718No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA15602.034269618965652No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA15021.9586365177476983No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA14551.8973476253814254No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA12711.6574081318623999No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA12501.6300237331455545No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA11961.5596067078736666No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA11131.4513731319928018No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA10701.3953003155725947No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA10561.3770440497613645No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10371.352267689017552No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA10211.331403385233289No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA10021.3066270244894767No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9731.2688104738804997No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9481.2362099992175886No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA9221.202305505568161No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA9191.1983934486086119No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA8861.155360822053569No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8801.1475367081344703No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA8681.131888480296273No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8411.0966799676603292No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7991.0419111702266386No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7530.9819262968468822No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7380.9623660120491354No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6860.8945570247502803No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6830.8906449677907311No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA6310.8228359804918759No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6290.8202279425188431No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6020.7850194298828991No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5870.7654591450851524No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5870.7654591450851524No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5800.7563310121795374No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5780.7537229742065045No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA5780.7537229742065045No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5710.7445948413008894No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5580.7276425944761755No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5370.7002581957593302No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4060.5294317085256761No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4000.5216075946065775No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3990.520303575620061No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3990.520303575620061No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3950.5150874996739953No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3900.508567404741413No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3810.496831233862765No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3740.4877031009571499No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3510.4577106642672717No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3250.42380617061784415No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3200.417286075685262No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2680.3494770883864069No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2630.3429569934538247No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2590.3377409175077589No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2570.335132879534726No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2550.33252484156169315No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2500.32600474662911094No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2470.3220926896695616No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2370.30905249980439714No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2360.3077484808178807No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2230.2907962339931669No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2200.28688417703361757No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA2190.2855801580471012No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2100.2738439871684532No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2100.2738439871684532No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2030.26471585426283806No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2010.2621078162898052No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1940.2529796833841901No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1800.23472341757295986No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1760.22950734162689407No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1700.22168322770779542No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1690.220379208721279No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1690.220379208721279No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1630.21255509480218032No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1620.21125107581566388No Hit
GGACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1610.2099470568291474No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1570.20473098088308167No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1500.19560284797746655No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1450.18908275304488434No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1420.185170696085335No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1410.18386667709881854No Hit
GGACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1400.1825626581123021No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1390.1812586391257857No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1390.1812586391257857No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.17995462013926922No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1380.17995462013926922No Hit
GGACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1350.1760425631797199No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1340.17473854419320345No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1290.16821844926062124No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1280.1669144302741048No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1280.1669144302741048No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1270.16561041128758833No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1250.16300237331455547No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1240.161698354328039No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1220.15909031635500614No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1220.15909031635500614No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1210.15778629736848968No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1200.15648227838197323No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1180.15387424040894035No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.1525702214224239No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1150.14996218344939102No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1130.14735414547635814No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1070.13953003155725946No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1050.1369219935842266No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1000.13040189865164437No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA980.12779386067861148No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA950.12388180371906216No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA910.11866572777299639No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA910.11866572777299639No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.11736170878647993No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA900.11736170878647993No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA900.11736170878647993No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA900.11736170878647993No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA890.11605768979996349No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA880.11475367081344703No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.11475367081344703No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA870.11344965182693059No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA860.11214563284041416No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA860.11214563284041416No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA840.10953759486738127No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA840.10953759486738127No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA810.10562553790783194No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA800.1043215189213155No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA780.10171348094828261No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.10171348094828261No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA770.10040946196176617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCC206.1476385E-8295.0295
GGACTAC72750.0295.01
TCGGGTT850.0295.08
ACAGGGG8550.0295.06
AATTGGC108.449649E-4295.0295
AGTTCTC108.449649E-4295.0295
AATTCGC400.0295.0295
AGTTAGA255.2568794E-10295.0295
AGGGGTA7500.0295.08
GATTACA108.449649E-4295.02
GGTAGCC108.449649E-4295.0295
TGACTAC108.449649E-4295.01
GTACTAC157.214083E-6294.999971
AGTTAGG157.214083E-6294.99997295
GGTTAGC157.214083E-6294.99997295
ACAAGGG15700.0294.060526
CAAGGGT15650.0294.057537
CAGGGGT8650.0293.29487
TACAGGG8600.0293.284885
CTACTAG8100.0293.179024
Produced by FastQC (version 0.11.5)