FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65789
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA650.0295.02
TCGCCCT303.6379788E-12295.01
AGATCCT206.140908E-8295.01
ACGCCTA1300.0295.02
GCGACCT303.6379788E-12295.01
GATCCTA700.0295.02
GGTCCTA600.0295.02
CGACCTA750.0295.02
TAACGGG108.4469194E-4295.07
CGTTCCT108.4469194E-4295.01
ACGGCGG108.4469194E-4295.08
GGGCGGC108.4469194E-4295.09
AGCGCCT303.6379788E-12295.01
GACGCCT400.0295.01
CGGTCCT206.140908E-8295.01
CGTCCTA850.0295.02
TTCGCCT255.2568794E-10295.01
CGATCCT206.140908E-8295.01
TCGACCT255.2568794E-10295.01
ACCTACG17500.0292.471444
Produced by FastQC (version 0.11.5)