FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52446
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC540.1029630477062121No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.10105632460054152No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC530.10105632460054152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCT157.2017247E-6294.993351
AGGAGGC157.2017247E-6294.993358
CCTAGGA157.2017247E-6294.993355
GCGCGAA600.0294.99335295
TGGCCCT157.2017247E-6294.993351
CCTCCCT303.6379788E-12294.993351
TCAGCCT157.2017247E-6294.993351
GTTCCTA600.0294.993352
GAGCCTA650.0294.993352
TTGCCCT303.6379788E-12294.993351
GGCGCCT157.2017247E-6294.993351
GGACCCT157.2017247E-6294.993351
TAGGAGG157.2017247E-6294.993357
CTCTCCT303.6379788E-12294.993351
TGGTCCT303.6379788E-12294.993351
TGCCGGG157.2017247E-6294.99335295
GCCCCTA350.0294.993322
TGCCCCT108.442601E-4294.993321
CGGCCTA850.0294.993322
GCGGTAG108.442601E-4294.99332295
Produced by FastQC (version 0.11.5)