FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80979
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCT350.0295.000031
AGATCCT255.275069E-10295.01
CACGGGA255.275069E-10295.06
GATCCTA750.0295.02
CGCGCCT255.275069E-10295.01
TCGTCCT450.0295.01
CTCGCCT255.275069E-10295.01
GATTCCT255.275069E-10295.01
ATCGCCT255.275069E-10295.01
TTGTCCT206.1496394E-8294.999971
GCGCCTA1050.0294.999972
CCTACGA108.4505253E-4294.999975
GCCGCCT157.215578E-6294.999971
TACGCCT108.4505253E-4294.999971
ATCCCCT303.6379788E-12294.999971
TAGTCCT400.0294.999971
GTAGTGG157.215578E-6294.99997295
ACCCTAC4500.0291.72223
GCCTACG14250.0289.824554
TCCTACG20650.0288.571444
Produced by FastQC (version 0.11.5)