FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75523
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA920.12181719476186063Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA890.1178448949326695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA860.11387259510347841Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA850.1125484951604147Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA830.10990029527428731Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA820.1085761953312236Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA780.1032797955589688Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA780.1032797955589688Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACACCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA780.1032797955589688Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCATCCTACGGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA760.10063159567284137Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA1050.0295.02
TGCCCCT450.0295.01
GCGCCCT108.449397E-4295.01
GGGCCTA800.0295.02
CCGACCT206.146911E-8295.01
AGGGCCT206.146911E-8295.01
CCGCCCT108.449397E-4295.01
GGGTCCT157.2136554E-6294.999971
CCGGCCT157.2136554E-6294.999971
GTGGCCT157.2136554E-6294.999971
TCGGCCT157.2136554E-6294.999971
CGCCCCT157.2136554E-6294.999971
CCTACGG65250.0290.252875
CTACGGT15250.0290.163946
GCCTACG14700.0289.9834
CCCTACG16300.0287.760744
TCCTACG17450.0287.392554
CTACGGG49700.0287.28376
ACGGGAG38250.0286.130748
GGCCTAC3150.0285.634923
Produced by FastQC (version 0.11.5)