FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75523
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3950.5230194775101625No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3890.5150748778517802No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3480.4607867801861685No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3350.4435734809263403No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2560.33896958542430783No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2520.333673185652053No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2320.30719118679077895No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2240.29659838724626936No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2010.2661440885558042No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1950.258199488897422No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1860.24628258940984868No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1700.2250969903208294No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.22244879043470203No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1590.2105318909471287No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1590.2105318909471287No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.18404989208585465No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.18272579214279094No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1310.17345709254134503No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.1668365928260265No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.1668365928260265No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1210.160216093110708No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1090.14432689379394356No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1000.13240999430637024No Hit
GGACTACCGTAGGATAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT960.12711359453411544Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT920.12181719476186063Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGATGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT880.11652079498960582Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT860.11387259510347841Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT850.1125484951604147Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA840.11122439521735102No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA800.1059279954450962No Hit
GGACTACCGTAGGTTAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT800.1059279954450962Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT790.1046038955020325Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT790.1046038955020325Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGTGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT790.1046038955020325Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.1032797955589688No Hit
GGACTACCGTAGGATTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT780.1032797955589688Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGACGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT780.1032797955589688Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGTAGGAAAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT770.10195569561590509Illumina Single End PCR Primer 1 (100% over 33bp)
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA760.10063159567284137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)