FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86594
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1420.16398364782779407No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1390.1605192045638266No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1310.15128068919324664No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1290.14897106035060168No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1280.14781624592927917No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1220.1408873594013442No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1210.13973254498002172No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1210.13973254498002172No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.13857773055869924No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.13857773055869924No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.13857773055869924No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1190.13742291613737673No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1190.13742291613737673No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1190.13742291613737673No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1190.13742291613737673No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1180.13626810171605422No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1180.13626810171605422No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.13511328729473174No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.13511328729473174No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.13511328729473174No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.13511328729473174No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1170.13511328729473174No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.13511328729473174No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.13511328729473174No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1160.13395847287340923No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1160.13395847287340923No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1150.13280365845208675No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1150.13280365845208675No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1150.13280365845208675No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1150.13280365845208675No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1130.13049402960944176No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1130.13049402960944176No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1120.12933921518811928No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1120.12933921518811928No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1120.12933921518811928No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1110.12818440076679677No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1110.12818440076679677No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1110.12818440076679677No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1100.1270295863454743No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1100.1270295863454743No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1090.12587477192415178No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1090.12587477192415178No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.12587477192415178No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.12587477192415178No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1090.12587477192415178No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.1247199575028293No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.1247199575028293No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1080.1247199575028293No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1070.1235651430815068No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1050.12125551423886181No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1050.12125551423886181No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1040.12010069981753932No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1040.12010069981753932No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1040.12010069981753932No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.11894588539621684No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.11894588539621684No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.11894588539621684No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.11894588539621684No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1030.11894588539621684No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.11894588539621684No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.11779107097489433No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1020.11779107097489433No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.11779107097489433No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.11779107097489433No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1010.11663625655357185No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.11663625655357185No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.11663625655357185No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.11548144213224935No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.11548144213224935No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11548144213224935No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11548144213224935No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11548144213224935No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11548144213224935No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.11548144213224935No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.11548144213224935No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.11548144213224935No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.11432662771092685No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.11432662771092685No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.11432662771092685No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.11432662771092685No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.11432662771092685No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.11432662771092685No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.11317181328960436No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.11317181328960436No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.11317181328960436No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.11317181328960436No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.11317181328960436No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.11317181328960436No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.11317181328960436No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.11317181328960436No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.11201699886828187No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.11201699886828187No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.11201699886828187No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.11201699886828187No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.11201699886828187No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.11201699886828187No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.11201699886828187No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.11201699886828187No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.11201699886828187No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.11086218444695939No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11086218444695939No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11086218444695939No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.11086218444695939No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11086218444695939No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11086218444695939No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.11086218444695939No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.11086218444695939No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.10970737002563688No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.10970737002563688No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.10970737002563688No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC950.10970737002563688No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.10970737002563688No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10855255560431437No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10855255560431437No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.10855255560431437No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.10855255560431437No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.10855255560431437No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.10739774118299189No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.10739774118299189No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.10739774118299189No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.10739774118299189No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.10739774118299189No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.10739774118299189No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC930.10739774118299189No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.1062429267616694No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.1062429267616694No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.1062429267616694No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.1062429267616694No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.1062429267616694No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.1062429267616694No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.10508811234034691No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10508811234034691No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.10508811234034691No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10508811234034691No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10508811234034691No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10508811234034691No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.10508811234034691No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.10508811234034691No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.10508811234034691No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.1039332979190244No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.1039332979190244No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.1039332979190244No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.1039332979190244No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
GATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
GTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
ACGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.10277848349770193No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.10277848349770193No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.10162366907637943No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10162366907637943No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10162366907637943No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.10162366907637943No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10046885465505692No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10046885465505692No Hit
TATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10046885465505692No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10046885465505692No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10046885465505692No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10046885465505692No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10046885465505692No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.10046885465505692No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.10046885465505692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCT450.0295.01
CGTTCCT255.275069E-10295.01
ATCCCCT450.0295.01
TGGTCCT350.0295.01
AATCCTA1750.0295.02
TCCTCCT600.0295.01
ACGCCTA900.0295.02
TTCACCT550.0295.01
TTGCAGG400.0295.0295
GGGTCCT206.152186E-8295.01
ACGCCCT303.6379788E-12295.01
CCTACGA206.152186E-8295.05
CCTACCT350.0295.01
GGCACCT400.0295.01
ATCTCCT350.0295.01
CACACCT400.0295.01
TATACCT350.0295.01
TGACCTA1150.0295.02
CTCCCTA1300.0295.02
CGCCCTA1000.0295.02
Produced by FastQC (version 0.11.5)