FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66854
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCTA950.0295.000032
TTGTCCT350.0295.000031
TCGCCTA1200.0295.02
AGATCCT108.447234E-4295.01
ACGCCTA650.0295.02
CCGGCCT157.209961E-6295.01
ACCGCCT206.141636E-8295.01
GCGCCCT157.209961E-6295.01
GGCCCTA1000.0295.02
AAGCCTA550.0295.02
GGGCCTA650.0295.02
CCGACCT108.447234E-4295.01
GACGCCT157.209961E-6295.01
GGCGCCT157.209961E-6295.01
CGCACCT157.209961E-6295.01
CGTCCTA800.0295.02
TAAGCCT255.2568794E-10295.01
CCGCCTA900.0295.02
TTCCTCG108.447234E-4295.03
CGCCTAC3350.0294.999973
Produced by FastQC (version 0.11.5)