FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54090
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA700.0295.000032
TGGACCT255.2386895E-10295.000031
AAGCCTA500.0295.000032
TACGCCT255.2386895E-10295.000031
TGCGCCT350.0295.000031
GAAACCT255.2386895E-10295.000031
TGCTCCT255.2386895E-10295.000031
TTGCCTA500.0295.000032
TCCCCTA500.0295.000032
ACCCCTA500.0295.000032
TAGTCCT350.0295.000031
AGGTCCT255.2386895E-10295.000031
CGGACCT255.2386895E-10295.000031
GCCCCTA600.0295.02
GTCGCCT157.2023286E-6295.01
ACGCCTA650.0295.02
ACGCCCT108.442764E-4295.01
GCGACCT157.2023286E-6295.01
GTCCTCG108.442764E-4295.03
GTCCTAC3750.0295.03
Produced by FastQC (version 0.11.5)