FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83514
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.02
TAGACCT157.216391E-6295.01
GATCCTA1200.0295.02
GCGCCTA450.0295.02
GCGCCCT303.6379788E-12295.01
TACGCCT303.6379788E-12295.01
CTCGGGA500.0295.06
CTCGCCT350.0295.01
TTCGCCT157.216391E-6295.01
ATCGCCT350.0295.01
GTGCCTA700.0295.02
GCCCCCT108.4510015E-4294.999971
GCGCGAA400.0294.99997295
CGATCCT400.0294.999971
CGCCCTA1050.0294.999972
TACGTGA108.4510015E-4294.999977
TCCTACG21250.0290.141174
CCTACGG75550.0288.94775
ACGGGAG72450.0288.688758
ACCTACG22900.0288.558934
Produced by FastQC (version 0.11.5)