FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005491540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83514
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT18732.242737744569773No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT13681.6380487103958619No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT10911.306367794621261No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG9701.161481907225136No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT7730.9255933136959074No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT7590.9088296573029672No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7350.8800919606293556No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6500.7783126182436478No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6080.7280216490648275No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5780.6920995282228128No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5730.6861125080824773No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5590.6693488516895372No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5580.6681514476614699No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA5310.6358215389036569No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC4970.5951098019493738No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4540.5436214287424862No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4450.5328447924898819No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4300.5148837320688747No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC4120.493330459563666No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA4030.48255382331106156No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC3680.44064468232871135No Hit
GGACTACAAGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG3650.43705247024450994No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3540.42388102593577126No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3360.40232775343056254No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3150.37718226884115236No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3030.36281342050434656No Hit
GGACTACAAGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT3010.36041861244821227No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2970.3556289963359437No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2940.35203678425174223No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2880.3448523600833393No Hit
GGACTACAAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG2790.33407572383073497No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2760.3304835117465335No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2700.32329908757813064No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2640.3161146634097277No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2630.3149172593816606No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC2560.3065354311851905No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2520.3017458150729219No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2450.2933639868764519No Hit
GGACTACTAGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG2440.2921665828483847No Hit
GGACTACTAGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT2400.2873769667361161No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2360.28258735062384754No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2330.2789951385396461No Hit
GGACTACAAGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG2310.27660033048351174No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2300.2754029264554446No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2270.2718107143712431No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2220.2658236942309074No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2190.26223148214670594No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2170.25983667409057165No Hit
GGACTACACGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG2140.2562444620063702No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2110.2526522499221687No Hit
GGACTACACGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT2040.24427042172569866No Hit
GGACTACTAGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG2040.24427042172569866No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1930.23109897741696003No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1920.2299015733888929No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1860.22271714922048996No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1830.21912493713628856No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1830.21912493713628856No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1810.2167301290801542No Hit
GGACTACAAGGGTTTCTAATCTTGGCTGCACACTAGCATCACTCTGGTTT1800.2155327250520871No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1780.21313791699595278No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1770.21194051296788563No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1770.21194051296788563No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1730.20715089685561702No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1710.20475608879948273No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1700.20355868477141556No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1680.20116387671528127No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1670.1999664726872141No Hit
GGACTACTCGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG1610.1927820485188112No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1560.18679502837847548No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1560.18679502837847548No Hit
GGACTACTAGGGTTTCTAATCTTGGCTGCACACTAGCATCACTCTGGTTT1540.18440022032234116No Hit
GGACTACAAGGGTTTCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC1530.183202816294274No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1510.18080800823813972No Hit
GGACTACTGGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG1490.17841320018200543No Hit
GGACTACAAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA1490.17841320018200543No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1480.17721579615393826No Hit
GGACTACTAGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1470.17601839212587111No Hit
GGACTACACGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1470.17601839212587111No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1460.17482098809780397No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1440.17242618004166965No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1370.1640443518451996No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1360.16284694781713246No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1360.16284694781713246No Hit
GGACTACAAGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG1350.16164954378906532No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1300.15566252364872954No Hit
GGACTACTGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT1290.1544651196206624No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1270.1520703115645281No Hit
GGACTACCGGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG1260.15087290753646096No Hit
GGACTACTAGGGTTTCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC1250.1496755035083938No Hit
GGACTACTCGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT1250.1496755035083938No Hit
GGACTACAAGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTGA1240.14847809948032664No Hit
GGACTACCAGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG1200.14368848336805806No Hit
GGACTACACGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1200.14368848336805806No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1190.14249107933999092No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1180.14129367531192377No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1180.14129367531192377No Hit
GGACTACAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT1170.1400962712838566No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1140.13650405919965514No Hit
GGACTACTCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1120.13410925114352085No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1120.13410925114352085No Hit
GGACTACAGGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG1110.1329118471154537No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1080.12931963503125224No Hit
GGACTACAAGGGTTTCTAATGGCACAGGGGCTGGAAGGATCCCATCTTCA1080.12931963503125224No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1070.1281222310031851No Hit
GGACTACTAGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTGA1060.12692482697511795No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1060.12692482697511795No Hit
GGACTACTGGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG1050.1257274229470508No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1040.12453001891898365No Hit
GGACTACTGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1040.12453001891898365No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1030.1233326148909165No Hit
GGACTACACGGGTTTCTAATCTTGGCTGCACACTAGCATCACTCTGGTTT1030.1233326148909165No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1020.12213521086284933No Hit
GGACTACAAGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1010.12093780683478218No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1000.11974040280671504No Hit
GGACTACTAGGGTTTCTAAGACTCTTGTCAACGTATAAGCCTTCCTGTTG1000.11974040280671504No Hit
GGACTACAGGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1000.11974040280671504No Hit
GGACTACAAGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG990.11854299877864789No Hit
GGACTACTCGGGTTTCTAATTCCACGTCACTAGAAAGTTCTTGGGCCCCG990.11854299877864789No Hit
GGACTACCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT990.11854299877864789No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC990.11854299877864789No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC990.11854299877864789No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC980.11734559475058075No Hit
GGACTACAAGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA980.11734559475058075No Hit
GGACTACCAGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT980.11734559475058075No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT980.11734559475058075No Hit
GGACTACACGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA980.11734559475058075No Hit
GGACTACCCGGGTTTCTAATTTTAAAACTGGTGGGTAGATTCCTGAAATG970.11614819072251359No Hit
GGACTACACGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTGA950.11375338266637929No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG950.11375338266637929No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA950.11375338266637929No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA940.11255597863831213No Hit
GGACTACTGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTGA940.11255597863831213No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC940.11255597863831213No Hit
GGACTACAAGGGTTTCTAATGGCAGTGACAATGACAGTGTCCTCTGCATC940.11255597863831213No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA930.11135857461024498No Hit
GGACTACCCGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATTT930.11135857461024498No Hit
GGACTACACGGGTTTCTAATTTTCCTCTCACCTCCCTACTGCTCCTTCTC920.11016117058217784No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA870.10417415044184208No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA870.10417415044184208No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.10417415044184208No Hit
GGACTACAAGGGTTTCTAATGGTCCCCGGAGTTTCTCGGCACAGAGCTCA870.10417415044184208No Hit
GGACTACTAGGGTTTCTAATACCTCTTGTCCATGTCTTATGATACGGCCA860.10297674641377492No Hit
GGACTACAAGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTAC860.10297674641377492No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA850.10177934238570778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)