FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82445
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCT255.275069E-10295.01
CCCCTAG108.4507983E-4295.03
ACCGCCT400.0295.01
TCTACGG108.4507983E-4295.04
CGCTACG108.4507983E-4295.03
GTGTCGG206.150367E-8295.0295
CTCGCCT350.0295.01
CGTACCT350.0295.01
GACGCCT350.0295.01
GGCGCCT255.275069E-10295.01
CCGCCTA1050.0295.02
ACCCCTA1000.0295.02
GCGTCCT157.2160456E-6295.01
ATCGCCT350.0295.01
ACGACCT303.6379788E-12295.01
TGATCTA303.6379788E-12295.0295
CCTACGG78650.0288.998725
TCCTACG21750.0288.896554
CTACGGG76550.0288.64146
TACGGGA76100.0287.634677
Produced by FastQC (version 0.11.5)