FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63240
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA700.0295.000032
CCGACCT350.0295.000031
TGATCCT108.4461446E-4295.01
AGATCCT206.139089E-8295.01
CGAACCT303.6379788E-12295.01
GTTTCCT400.0295.01
TAAAGCC108.4461446E-4295.0295
GACACCT157.2080984E-6295.01
GTCCTAC4000.0295.03
GATCCTA500.0295.02
GGTCCTA1000.0295.02
TAGCCTA850.0295.02
GACCCTA800.0295.02
CGTTCCT157.2080984E-6295.01
TACGCCT108.4461446E-4295.01
CGGTCCT255.2568794E-10295.01
GGCGCCT255.2568794E-10295.01
GCTACGG157.2080984E-6295.04
TCCCACG108.4461446E-4295.03
ATCGCCT206.139089E-8295.01
Produced by FastQC (version 0.11.5)