FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79534
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.1345336585611185No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.12070309553147082No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11944577161968467No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.1181884477078985No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11693112379611235No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.11567379988432619No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11441647597254005No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11441647597254005No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11441647597254005No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.11441647597254005No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11190182814896774No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11190182814896774No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11190182814896774No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.11064450423718158No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10938718032539542No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10812985641360927No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10687253250182313No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10561520859003695No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.10561520859003695No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10435788467825081No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10435788467825081No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.10435788467825081No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10310056076646466No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10310056076646466No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10310056076646466No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10310056076646466No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10310056076646466No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1018432368546785No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1018432368546785No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1018432368546785No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10058591294289235No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.10058591294289235No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.10058591294289235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA850.0295.000032
TCCCCTA850.0295.000032
CGTTCCT303.6379788E-12295.01
ATCCCCT255.275069E-10295.01
TGGTCCT350.0295.01
TCCTCGG108.450239E-4295.04
AACGCCT350.0295.01
TCCTCCT303.6379788E-12295.01
GTGCATA108.450239E-4295.0295
GGGTCCT157.2150906E-6295.01
CCTACGA255.275069E-10295.05
GTCACCT500.0295.01
GGCACCT206.148912E-8295.01
CACACCT303.6379788E-12295.01
TCGACCT108.450239E-4295.01
CTCCCTA1050.0295.02
GAAACCT500.0295.01
AAATCCT108.450239E-4295.01
TTCGCCT350.0295.01
TGCGCCT350.0295.01
Produced by FastQC (version 0.11.5)