FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78694
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1500.19061173659999492No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1210.15376013419066256No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1140.14486491981599614No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1140.14486491981599614No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1140.14486491981599614No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1130.14359417490532952No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1130.14359417490532952No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1100.13978194017332962No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1080.13724045035199633No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.13342821561999643No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.13342821561999643No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1040.13215747070932982No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1040.13215747070932982No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1030.13088672579866317No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1010.1283452359773299No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1010.1283452359773299No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1000.12707449106666327No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1000.12707449106666327No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1000.12707449106666327No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC990.12580374615599665No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC990.12580374615599665No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC980.12453300124533001No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC980.12453300124533001No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC980.12453300124533001No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC980.12453300124533001No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC970.12326225633466337No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC970.12326225633466337No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.12199151142399675No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.12199151142399675No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.12199151142399675No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC960.12199151142399675No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.12072076651333012No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.12072076651333012No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC950.12072076651333012No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC940.11945002160266348No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.11817927669199686No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.11817927669199686No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.11817927669199686No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC930.11817927669199686No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.11690853178133022No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.11690853178133022No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC910.11563778687066358No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC910.11563778687066358No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.11436704195999695No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.11436704195999695No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.11436704195999695No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.11436704195999695No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC900.11436704195999695No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11309629704933033No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11309629704933033No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11309629704933033No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.11309629704933033No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.11182555213866369No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.11182555213866369No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.11182555213866369No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.11182555213866369No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC880.11182555213866369No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11055480722799704No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC870.11055480722799704No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
TGGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.10928406231733043No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.10801331740666378No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.10801331740666378No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.10801331740666378No Hit
CAGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.10801331740666378No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10674257249599715No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC840.10674257249599715No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.10547182758533051No Hit
GAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.10547182758533051No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.10547182758533051No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
AGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
TTCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC820.1042010826746639No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10293033776399725No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10293033776399725No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10293033776399725No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10293033776399725No Hit
CTCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10293033776399725No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10293033776399725No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10165959285333062No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10165959285333062No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10165959285333062No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.10165959285333062No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10038884794266399No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10038884794266399No Hit
ACAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10038884794266399No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10038884794266399No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10038884794266399No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10038884794266399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.000032
GTCCTAC5300.0295.03
GATCCTA1350.0295.02
CGGGGCA157.2147977E-6295.09
AACGCCT157.2147977E-6295.01
GCTACCT303.6379788E-12295.01
CGATCCT550.0295.01
CCCCTCG108.450069E-4294.999973
CCCTCGG108.450069E-4294.999974
GCGACCT108.450069E-4294.999971
CAACGGG108.450069E-4294.999976
CCGGCCT206.148548E-8294.999971
CGCGCCT108.450069E-4294.999971
CGTTCCT206.148548E-8294.999971
TACGCCT206.148548E-8294.999971
GTGTCGG206.148548E-8294.99997295
CGGGCCT206.148548E-8294.999971
GGAACCT206.148548E-8294.999971
CGGGAGT108.450069E-4294.999979
CCGACCT400.0294.999971
Produced by FastQC (version 0.11.5)