FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78694
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA20372.588507383027931No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA18772.38518819732127No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15001.9061173659999493No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13291.6888199862759552No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13281.6875492413652882No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13121.6672173227946223No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12661.608763056903957No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12061.532518362263959No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11301.435941749053295No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10871.3812997178946298No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9681.2300810735253005No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8611.094111368083971No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8361.062342745317305No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8171.038198592014639No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7730.9822858159453071No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6710.8526698350573106No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6360.8081937631839784No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT5140.6531628840826493No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4940.6277479858693166No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4600.584542658906651No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4370.5553155259613185No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4350.5527740361399852No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3940.5006734948026533No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3600.4574681678399878No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3570.45365593310798785No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3270.41553358578798893No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3260.41426284087732224No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC3260.41426284087732224No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3120.39647241212798945No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2950.3748697486466567No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2920.3710575139146568No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2890.36724527918265687No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2880.3659745342719902No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2850.36216229953999035No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2850.36216229953999035No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2850.36216229953999035No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2790.35453783007599055No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2790.35453783007599055No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2760.3507255953439906No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2730.34691336061199074No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2700.34310112587999086No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2660.33801814623732435No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2610.3316644216839912No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2580.32785218695199125No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2540.3227692073093247No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2530.3214984623986581No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2530.3214984623986581No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2500.3176862276666582No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2490.3164154827559916No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2460.3126032480239917No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2370.30116654382799196No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2370.30116654382799196No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2320.2948128192746588No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2320.2948128192746588No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2300.2922713294533255No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2270.28845909472132564No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC2190.27829313543599254No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2160.27448090070399267No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2140.2719394108826594No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2130.2706686659719928No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2130.2706686659719928No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2130.2706686659719928No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2110.2681271761506595No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2090.2655856863293263No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2030.25796121686532647No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2030.25796121686532647No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2010.2554197270439932No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1860.23635855338399372No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.23381706356266044No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1830.23254631865199377No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1800.2287340839199939No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1710.21729737972399418No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1690.21475588990266095No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1670.2122144000813277No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1660.21094365517066102No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1650.2096729102599944No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1630.20713142043866112No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1620.2058606755279945No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1610.2045899306173279No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1580.20077769588532798No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1580.20077769588532798No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1570.19950695097466134No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1550.1969654611533281No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1550.1969654611533281No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1530.19442397133199482No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1500.19061173659999492No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1460.1855287569573284No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1450.18425801204666176No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1420.18044577731466185No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1410.1791750324039952No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1410.1791750324039952No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1410.1791750324039952No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.17663354258266195No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1390.17663354258266195No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1370.1740920527613287No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.17155056293999543No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1350.17155056293999543No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1340.1702798180293288No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1290.16392609347599563No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1290.16392609347599563No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1250.1588431138333291No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.1588431138333291No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.15757236892266246No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1230.15630162401199585No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1230.15630162401199585No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1220.1550308791013292No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1210.15376013419066256No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1190.1512186443693293No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1170.14867715454799602No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1160.1474064096373294No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1140.14486491981599614No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1090.13851119526266298No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1050.13342821561999643No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1030.13088672579866317No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1020.12961598088799656No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1020.12961598088799656No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCA1020.12961598088799656No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC980.12453300124533001No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.12326225633466337No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT960.12199151142399675No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC960.12199151142399675No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.12072076651333012No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC940.11945002160266348No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.11817927669199686No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC930.11817927669199686No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA930.11817927669199686No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.11563778687066358No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC900.11436704195999695No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.11182555213866369No Hit
GGACTACACGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC860.10928406231733043No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA850.10801331740666378No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCA850.10801331740666378No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC840.10674257249599715No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCTTCA840.10674257249599715No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT830.10547182758533051No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.10547182758533051No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA820.1042010826746639No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGACATAATCTCATT810.10293033776399725No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA810.10293033776399725No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC800.10165959285333062No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA790.10038884794266399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGGT14100.0295.07
AATTAGC350.0295.0295
AGTTAGG108.450069E-4294.99997295
AGTAGCC108.450069E-4294.99997295
GGACTAC75950.0294.223181
TACAAGG14200.0293.961275
AAGGGTA8600.0293.284888
ACAAGGG14250.0292.929846
CTACCGG5850.0292.478644
CCGGGTA3900.0291.217938
CGGGGTA3550.0290.845068
TACTCGG7100.0290.845065
GACTACC21650.0290.230962
CTACAAG14450.0289.896184
GGGGTAT11400.0289.824559
TCGGGTT2550.0289.21578
CTACTCG7150.0288.81124
GACTACA35750.0288.81122
CGGGTAT17250.0288.159429
ACCCGGG7250.0286.86216
Produced by FastQC (version 0.11.5)