FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81007
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA1000.0295.02
TGCCACG108.450533E-4295.03
GAGTAAT108.450533E-4295.0295
CACGCCT206.1496394E-8295.01
CGCGCCT303.6379788E-12295.01
GCGCCTA1200.0295.02
ATCACGC108.450533E-4295.0295
TTCCCTA1150.0295.02
TACGCCT400.0295.01
GGGACCT303.6379788E-12295.01
TCTACGG157.2155926E-6295.04
AACGCCT303.6379788E-12295.01
CGGGCCT255.275069E-10295.01
AGCGCCT206.1496394E-8295.01
TACCTCG108.450533E-4295.03
GTTCCCT108.450533E-4295.01
GGTGCCT108.450533E-4295.01
GGGCCCT350.0295.01
CCGACCT108.450533E-4295.01
GTCCCTA550.0295.02
Produced by FastQC (version 0.11.5)