FastQCFastQC Report
Wed 8 May 2024
EGAF00005491565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108642
Total Bases32.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1430.13162497008523408No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1410.12978406141271331No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1340.12334088105889066No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1240.1141363376962869No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1230.11321588336002651No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1230.11321588336002651No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1220.11229542902376614No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1200.11045452035124538No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1190.109534066014985No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1190.109534066014985No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1190.109534066014985No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1160.10677270300620387No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1140.1049317943336831No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1140.1049317943336831No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1140.1049317943336831No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1130.10401133999742272No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1130.10401133999742272No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1110.10217043132490196No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1100.10124997698864159No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1100.10124997698864159No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCC1090.10032952265238121No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)