FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79505
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA21772.738192566505251No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16242.042638827746683No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14561.8313313628073706No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12941.6275705930444626No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12441.564681466574429No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11881.494245644927992No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA11631.4628010816929753No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11451.4401609961637634No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10881.3684673919879253No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9991.256524746871266No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9561.2024400981070373No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA9001.1320042764606No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8811.1081064084019872No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8781.1043330608137853No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA8261.0389283692849507No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8141.0238349789321426No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7760.9760392428149174No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7690.9672347651091127No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7030.8842211181686686No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7000.8804477705804666No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6970.8766744229922647No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6880.8653543802276586No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6660.8376831645808439No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6510.818816426639834No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6510.818816426639834No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6350.7986919061694233No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6350.7986919061694233No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6190.7785673856990126No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6130.7710206905226087No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6110.7685051254638073No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6090.765989560405006No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5920.7446072574051946No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5850.73580277969939No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5640.7093893465819759No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5530.6955537387585686No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5170.6502735677001447No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5010.630149047229734No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4750.5974467014653166No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4650.58486887617131No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4620.581095528583108No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4580.5760643984655053No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4480.5634865731714986No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4470.562228790642098No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4400.5534243129362933No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4340.5458776177598893No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4110.516948619583674No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3990.501855229230866No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3860.4855040563486573No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3680.4628639708194453No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3660.460348405760644No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3650.4590906232312433No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3610.4540594931136407No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3550.44651279793723664No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3520.44273945034903467No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3480.437708320231432No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3430.4314194075844287No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3290.4138104521728193No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3180.399974844349412No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3170.3987170618200113No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3160.3974592792906106No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3090.388654801584806No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3050.38362367146720333No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2980.37481919376139866No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2970.373561411231998No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2960.3723036287025973No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2830.35595245582038865No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2810.3534368907615873No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2780.3496635431733853No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2750.34589019558518336No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2640.332054587761776No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2630.3307968052323753No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2530.3182189799383687No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2450.30815671970316333No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2350.2955788944091567No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2110.26539211370354066No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2010.252814288409534No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1960.24652537576253064No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1920.241494245644928No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1910.24023646311552732No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1860.233947550468524No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1850.23268976793912335No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1780.22388529023331863No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1780.22388529023331863No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1770.22262750770391798No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1620.20376076976290797No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1590.199987422174706No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1570.19747185711590465No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1570.19747185711590465No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1490.18740959688069933No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1490.18740959688069933No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1490.18740959688069933No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1440.18112068423369598No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1380.173573989057292No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1260.158480598704484No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1260.158480598704484No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1230.154707251116282No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1210.15219168605748065No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1210.15219168605748065No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1190.14967612099867933No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1160.14590277341047733No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1150.14464499088107666No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1140.143387208351676No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1060.13332494811647067No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1030.12955160052826867No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA990.124520470410666No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC990.124520470410666No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA970.12200490535186467No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC940.11823155776366266No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA940.11823155776366266No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA940.11823155776366266No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT940.11823155776366266No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC920.11571599270486133No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA920.11571599270486133No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA910.11445821017546066No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA890.11194264511665931No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA880.11068486258725867No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA870.109427080057858No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC870.109427080057858No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA870.109427080057858No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC840.105653732469656No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA840.105653732469656No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA820.10313816741085466No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA810.10188038488145398No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC810.10188038488145398No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA810.10188038488145398No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC800.10062260235205332No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA800.10062260235205332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT108.450239E-4295.06
CGTTAGC500.0295.0295
CGGGGTA3550.0295.08
ACTCGGT108.450239E-4295.06
GGACTAC76200.0294.612851
GACTACC21550.0294.315522
TACTAGG9600.0293.463565
GACTACA18000.0293.361082
CTACCCG8350.0293.233554
ACACGGG7550.0293.046366
ACTACCG6800.0292.83093
GGGGTAT12600.0292.658759
ACTACTA9800.0291.98983
CAGGGGT4750.0291.89477
GACTACT37350.0291.840732
ACGGGTA4350.0291.60928
TACACGG7600.0291.11845
CTACCAG6550.0290.49624
AGGGTAT12250.0290.18379
ACTACAA5800.0289.91383
Produced by FastQC (version 0.11.5)