FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81019
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.02
GCCCCTA1200.0295.02
CGAACCT206.1496394E-8295.01
GTTTCCT108.450533E-4295.01
CCGGCCT303.6379788E-12295.01
CGCGCCT206.1496394E-8295.01
GGATCCT400.0295.01
GCGCCCT350.0295.01
ACGGGGC108.450533E-4295.07
CGGGGCA108.450533E-4295.08
TACGCCT255.275069E-10295.01
TGCGCCT350.0295.01
TCTACGG108.450533E-4295.04
AGACCCT350.0295.01
GGGCCTA800.0295.02
CTCGCCT206.1496394E-8295.01
CCGACCT255.275069E-10295.01
GGACCTA1250.0295.02
CGGTCCT450.0295.01
GGCGCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)