FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75523
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC760.10063159567284137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.194182
GCCCCTA700.0295.194182
AGTCCTA1400.0295.194182
GCGACCT255.2386895E-10295.194181
TGGACCT350.0295.194181
GATCCTA1300.0295.194182
AAGACCT500.0295.194181
CGTTCCT350.0295.194181
TGACCTA1250.0295.194182
TTGCCTA650.0295.194182
CGCACCT255.2386895E-10295.194181
AGGTCCT500.0295.194181
GTCGCCT206.12672E-8295.194151
TGCCCCT303.6379788E-12295.194151
TCCGCCT400.0295.194151
GGATCCT303.6379788E-12295.194151
GCGCCTA750.0295.194152
GCGCCCT108.432754E-4295.194151
GCACCCT157.1947125E-6295.194151
TCTACCT108.432754E-4295.194151
Produced by FastQC (version 0.11.5)