FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107156
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCT350.0295.000031
GTAGCCT350.0295.000031
ATCCCCT350.0295.000031
CATACCT700.0295.000031
GACGCCT350.0295.000031
CGTCCTA1400.0295.000032
CATCCCT350.0295.000031
ATGCCCT350.0295.000031
TGATCCT500.0295.01
TCGCCTA1550.0295.02
GTTGATT255.275069E-10295.0295
GCCCCTA1350.0295.02
AGTCCTA1700.0295.02
ATCTCCT550.0295.01
GTCGCCT206.158916E-8295.01
AGTCCCT255.275069E-10295.01
CCCCTAC5950.0295.03
CGAACCT400.0295.01
CCCTCCT450.0295.01
GTACAGG108.4543385E-4295.0295
Produced by FastQC (version 0.11.5)