FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70756
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.11447792413364238No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC790.11165130872293515No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC770.10882469331222792No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC740.1045847701961671No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.1017581547854599No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.1017581547854599No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC720.1017581547854599No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10034484708010627No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC710.10034484708010627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA950.0295.000032
ACAACCT550.0295.01
AATCCTA1300.0295.02
GTCGCCT255.2568794E-10295.01
TGCCCCT255.2568794E-10295.01
AGATCCT255.2568794E-10295.01
ACGCCCT500.0295.01
GCGACCT206.1441824E-8295.01
GTTTCCT255.2568794E-10295.01
CAGCCTA700.0295.02
GAGTCCT108.448276E-4295.01
GACACCT255.2568794E-10295.01
TGGACCT206.1441824E-8295.01
GCGCGAA800.0295.0295
GCAACCT350.0295.01
ATTACAG108.448276E-4295.0295
GTCTCCT255.2568794E-10295.01
CGACCTA1150.0295.02
CACGCCT350.0295.01
TCCTCGG206.1441824E-8295.04
Produced by FastQC (version 0.11.5)