FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62640
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10217113665389528No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10217113665389528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA600.0295.02
GCCCCCT206.1385435E-8295.01
GTCGCCT157.207773E-6295.01
AGATCCT206.1385435E-8295.01
CGAACCT206.1385435E-8295.01
ACGCCTA950.0295.02
CAACGGG108.4459526E-4295.06
GAGTCCT255.2568794E-10295.01
CAGCCCT255.2568794E-10295.01
GTCCTAC3450.0295.03
TTGTCCT350.0295.01
CGACCTA600.0295.02
CGCGCCT303.6379788E-12295.01
GACCCTA600.0295.02
CGTTCCT206.1385435E-8295.01
TAGCCCT157.207773E-6295.01
GCACCCT206.1385435E-8295.01
CGCCTCG108.4459526E-4295.03
GTTACCT350.0295.01
AGTGCCT157.207773E-6295.01
Produced by FastQC (version 0.11.5)