FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66371
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.15820162420334183No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC890.13409471003902307No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.12204125295686369No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.12053457082159377No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.12053457082159377No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.11752120655105391No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.11752120655105391No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.116014524415784No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.10998779587470431No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10848111373943439No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10848111373943439No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.10848111373943439No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10697443160416448No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10697443160416448No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10697443160416448No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10697443160416448No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10396106733362462No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10396106733362462No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.1024543851983547No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.1024543851983547No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.1024543851983547No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.1024543851983547No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10094770306308479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAC4100.0295.000033
TCGCCTA1100.0295.02
AGTCCTA650.0295.02
AAATCCT500.0295.01
GCGACCT255.2568794E-10295.01
TCCGCCT450.0295.01
CAGCCTA1000.0295.02
GTCTCCT350.0295.01
AAGACCT350.0295.01
GCGCCTA750.0295.02
TAGCCTA900.0295.02
TATGCCT255.2568794E-10295.01
GACCCTA950.0295.02
CTGACCT450.0295.01
CCGTCCT255.2568794E-10295.01
CGCCTAC3350.0295.03
TCAGCCT450.0295.01
CTGCCTA700.0295.02
ATCCCTA1000.0295.02
GTCACCT550.0295.01
Produced by FastQC (version 0.11.5)