FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90968
Sequences flagged as poor quality0
Sequence length301
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG157.218883E-6294.996156
ACCGCCT206.154187E-8294.996151
CGCGCCT350.0294.996151
GGATCCT450.0294.996151
CCTACGC108.4525696E-4294.996155
GCGCCTA1200.0294.996152
TACGGGT108.4525696E-4294.996157
CGCGAGG108.4525696E-4294.996159
CGGGGCA108.4525696E-4294.996159
GAGCCTA1350.0294.996152
CGGTCCT255.275069E-10294.996151
GGCGCCT157.218883E-6294.996151
GGACCCT450.0294.996151
CGATCCT206.154187E-8294.996151
ACGACCT206.154187E-8294.996151
CTAGCCT400.0294.996151
TCCTACG23700.0292.506744
GTCCTAC4850.0291.954963
CCTACGG84250.0291.494725
CCCTACG19650.0290.49244
Produced by FastQC (version 0.11.5)