FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70355
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.1066022315400469No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.10518086845284627No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.10375950536564565No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.10375950536564565No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC720.10233814227844504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTA1400.0295.000032
GTCGCCT350.0295.000031
CCCCTAC4150.0295.000033
CGCGCCT350.0295.000031
GCGCCTA850.0295.000032
CATACCT350.0295.000031
GGGCCTA700.0295.000032
ACTCCTA1400.0295.000032
CGCACCT350.0295.000031
CGCCCTA700.0295.000032
GCCCCTA1050.0295.02
GTTGAGA108.448175E-4295.0295
CGAACCT303.6379788E-12295.01
ACGCCTA650.0295.02
CCTAGGG303.6379788E-12295.05
TGGACCT650.0295.01
ATTACCT400.0295.01
TTGTCCT206.1440005E-8295.01
GGATCCT157.2115636E-6295.01
TGAACCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)