FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73501
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA650.0295.02
CAGCCTA1150.0295.02
CGGCCTA750.0295.02
AGGACCT255.2568794E-10295.01
ACCCTAC4700.0295.03
TAGCCCT255.2568794E-10295.01
ACGGGGC303.6379788E-12295.08
AGAACCT255.2568794E-10295.01
GCACCTA850.0295.02
ATTCCTA1350.0295.02
CGGGGCA303.6379788E-12295.09
TGACCTA1300.0295.02
GGGACCT255.2568794E-10295.01
CGCTCCT450.0295.01
CTACGAG303.6379788E-12295.06
CTGCCTA500.0295.02
GTCACCT157.212877E-6295.01
CGGGCCT255.2568794E-10295.01
CCCCCTA900.0295.02
TCGTCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)