FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005491607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005491607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173754
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACG44850.0290.390934
CCTACGG166400.0288.790285
ACGGGAG157500.0287.784128
CTACGGG163150.0287.220186
ACCTACG50650.0285.38524
TACGGGA159600.0285.383737
CGGGAGG154950.0284.428969
GCCTACG31700.0283.82814
GTCCTAC10450.0282.291963
CCCTACG42250.0278.237984
CGCCTAC9200.0274.153083
CGCGCCT600.0270.41221
GCGCCTA2350.0269.889132
CGCCCTA2900.0269.564482
TCCCTAC10900.0269.284523
GACCTAC12050.0268.06613
CTCCTAC10900.0267.931343
GGCCTAC8000.0265.49563
TCGCCTA2500.0265.495572
ACCCTAC11800.0264.99563
Produced by FastQC (version 0.11.5)